Affinity changes for Na+ and K+ and three dimensional structural changes in the Na+ pump
نویسندگان
چکیده
منابع مشابه
Structural and functional organization of the Na+, K+-pump.
The active transport of Na’ and K + across cell membranes is catalysed by the ubiquitous transport system known as the Na+,K+-pump. Historically, Dean [ l] was the first to propose the name sodium pump for this physiological process, and in 1057 Skou discovered the enzyme, the Na+ ,K +-activated ATPase, soon shown to be identical to the transport system [2]. The structure and mechanism of Na+,K...
متن کاملChanges in affinity of Na+- and K+-transport ATPase for divalent cations during its reaction sequence.
Previous experiments (Fukushima, Y., and Post, R.L. (1978) J. Biol. Chem. 253, 6853-6862) demonstrated that the Ca x phosphoenzyme of sodium- and potassium-transport adenosine triphosphatase gradually becomes stable after dissociation of Ca2+ in the presence of a chelating agent such as 1,2-cyclohexylenedinitrilo-tetraacetic acid. In the present study, we investigated whether the ADP- and K+-se...
متن کاملAldosterone regulation of intestinal Na absorption involves SGK-mediated changes in NHE3 and Na+ pump activity.
Aldosterone-induced intestinal Na(+) absorption is mediated by increased activities of apical membrane Na(+)/H(+) exchange (aNHE3) and basolateral membrane Na(+)-K(+)-ATPase (BLM-Na(+)-K(+)-ATPase) activities. Because the processes coordinating these events were not well understood, we investigated human intestinal Caco-2BBE cells where aldosterone increases within 2-4 h of aNHE3 and alpha-subu...
متن کاملNa and K dependence of the Na/K pump in cystic fibrosis fibroblasts.
The Na and K dependence of the Na/K pump was measured in skin fibroblasts from patients with cystic fibrosis and age/sex-matched controls. Under basal conditions, there was no difference between control and cystic fibrosis cells in protein per cell, intracellular Na and K content, or Na/K pump activity (measured as ouabain-sensitive 86Rb uptake). There was no difference in the Na dependence of ...
متن کاملErythrocyte Na+,K+ cotransport and Na+,K+ pump in black and caucasian hypertensive patients.
Alterations in red blood cell (RBC) Na+,K+ pump and in Na+,K+ cotransport (CoT) have been described in essential hypertension (EH). We examined pump and CoT in 50 normotensive (NT) subjects and 58 EH subjects subdivided by race and family history of hypertension (+ FH). RBCs were preloaded with Na+ to obtain intracellular levels of 25 mM/liter cells by using the p-chloromercuribenzene sulfonic ...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1987
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)58083-0